Construction of a DNA database for ticks collected in Japan: application of molecular identification based on the mitochondrial 16S rDNA gene

Autor: Yasuhiro Yano, Miyako Tsurumi, Nobuhiro Takada, Shuji Ando, Teruki Kadosaka, Hiroki Kawabata, Takeo Yamauchi, Yosaburo Oikawa, Mutsuyo Gokuden, Hiromi Fujita, Mamoru Takahashi, Kozue Sato, Fubito Ishiguro, Masako Andoh, Ai Takano, Toshirou Honda, Takashi Tsunoda
Rok vydání: 2014
Předmět:
Zdroj: Medical Entomology and Zoology. 65:13-21
ISSN: 2185-5609
0424-7086
Popis: Tick identification is important in control of tick-born diseases because tick-borne pathogens are often transmitted by specific tick species. In this study, we determined partial DNA sequences of the mitochondrial 16S rDNA gene (mt-rrs ) for ticks including 7 genera and 39 species, and these ticks were allocated to 113 sequence types. Of the 39 species of ticks, 36 species (92.3%) were distinguishable by phylogenetic analysis of mt-rrs . This result suggests that species identification of ticks based on mt-rrs is a viable alternative to morphological identification. In order to establish a DNA database for identification of ixodid and argasid ticks in Japan, we deposited all sequence data in GenBank (from AB819156 to AB819268).
Databáze: OpenAIRE