Nonlinear absorption extends confocal fluorescence microscopy into the ultra-violet regime and confines the illumination volume
| Autor: | Georg Ritter, Ernst H. K. Stelzer, Nick Salmon, Steffen Lindek, Rainer Pick, Reinhold Stricker, Stefan W. Hell, Clemens Storz |
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| Rok vydání: | 1994 |
| Předmět: |
Materials science
Super-resolution microscopy business.industry Confocal Bright-field microscopy Scanning confocal electron microscopy Physics::Optics Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Optics Two-photon excitation microscopy Light sheet fluorescence microscopy Microscopy 4Pi microscope Electrical and Electronic Engineering Physical and Theoretical Chemistry business |
| Zdroj: | Optics Communications. 104:223-228 |
| ISSN: | 0030-4018 |
| DOI: | 10.1016/0030-4018(94)90546-0 |
| Popis: | It is shown that two-photon absorption confines the illumination volume and present quantitative evidence that an additional confocal arrangement of the detector further improves the resolution by 48%. The axial resolution in a confocal fluorescence microscope using two-photon absorption with infra-red light is comparable to that achievable with ultra-violet light half the wavelength. An important advantage of two-photon microscopy over single-photon microscopy is that absorption is almost confined to the observed volume. This means no photodamage is caused outside the observed volume. |
| Databáze: | OpenAIRE |
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