A New Rapid Method for the Determination of Serum Lipase

Autor:	Charles Sobel, Philip H. Dirstine, Richard J. Henry
Rok vydání:	1968
Předmět:	Zero order Chromatography biology Biochemistry (medical) Clinical Biochemistry Kinetics chemistry.chemical_element Normal values Copper Incubation period chemistry biology.protein Serum lipase Enzyme kinetics Lipase
Zdroj:	Clinical Chemistry. 14:1097-1106
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/14.11.1097
Popis:	A new method is described for the determination of serum lipase using olive oil as substrate, and photometric, copper soap analysis for the measurement of fatty acids formed. The great sensitivity of this technic for measurement of the fatty acids permits a 30-min. incubation time and the use of 0.1 ml. serum. The reaction is zero order for this time period. Other aspects of the enzyme kinetics were studied and the normal values determined.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c35b14df3329d649ae85259f702680ca https://doi.org/10.1093/clinchem/14.11.1097 Zobrazit plný text záznamu