Influence of Blue-Light Signaling Components on the Regulation of Cytokinin-Dependent Arabidopsis thaliana Seedlings’ Greening
| Autor: | A. S. Doroshenko, Victor V. Kusnetsov, A. S. Medvedeva, M. N. Danilova |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology food and beverages Plant physiology Plant Science biology.organism_classification 01 natural sciences Cell biology Chloroplast 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Greening chemistry Chlorophyll Etiolation Cytokinin Arabidopsis thaliana Photomorphogenesis 010606 plant biology & botany |
| Zdroj: | Russian Journal of Plant Physiology. 66:864-871 |
| ISSN: | 1608-3407 1021-4437 |
| DOI: | 10.1134/s1021443719060050 |
| Popis: | De-etiolation of plant seedlings is controlled by a complex system of light and hormonal signaling. This process is accompanied by chloroplast development and initiation of chlorophyll biosynthesis leading to cotyledon greening. In this study, peculiaritis of regulation of expression of the key genes encoding enzymes of chlorophyll biosynthesis in wild type plants and mutants of perception and transduction of blue light in Arabidopsis thaliana (L.) Heynh. during cytokinin (CK)-dependent greening of seedlings were investigated. Inactivation of blue-light signaling components (СRY1, CRY2 and HY5) led to retarded chlorophyll accumulation and decrease of the expression of the key genes responsible for its biosynthesis. Exogenous application of cytokinin accelerated de-etiolation of wild type and knockout-mutants cry1cry2 and hy5 seedlings that was manifested itself in elevating chlorophyll a fluorescence and higher level of transcripts of a number of genes involved in chlorophyll biosynthesis. Taken together the results may provide evidence for the significant contribution of blue-light signaling to the greening regulation at early steps of de-etiolation of seedlings and involvement of HY5 protein in realization of the positive effect of CK at early steps of photomorphogenesis. |
| Databáze: | OpenAIRE |
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