Saccharification of modified starch to maltose in a semi-continuous counter-current chromatographic reactor-separator (SCCR-S)
| Autor: | P. E. Barker, M. T. Shieh |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Renewable Energy Sustainability and the Environment Starch General Chemical Engineering Organic Chemistry Maltose Pollution Enzyme assay Modified starch Inorganic Chemistry chemistry.chemical_compound Hydrolysis Fuel Technology Column chromatography chemistry Bioreactor biology.protein Dextrin Waste Management and Disposal Biotechnology |
| Zdroj: | Journal of Chemical Technology AND Biotechnology. 63:125-134 |
| ISSN: | 1097-4660 0268-2575 |
| DOI: | 10.1002/jctb.280630206 |
| Popis: | Combined bioreaction and separation has been successfully carried out in a Simulated Counter-Current Chromatographic Reactor–Separator (SCCR–S) system for the saccharification of modified starch to maltose and dextrin. The effects of the operating parameters (switch time, eluent flowrate, feed concentration and enzyme activity) on the performance of the SCCR–S system were investigated. By using an eluent of dilute enzyme solution, starch conversions of up to 60% were achieved using lower amounts of enzyme than the theoretical amount required by a conventional bioreactor to produce the same amount of maltose over the same time period. Comparing the SCCR–S system with a continuous rotating annular chromatograph (CRAC) for the saccharification of modified starch showed that the SCCR–S system required only 34·6–47·3% of the amount of enzyme required by the CRAC, system. |
| Databáze: | OpenAIRE |
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