Isolation and characterization ofE. colistrains causing intramammary infections from dairy animals and wild birds
| Autor: | Elshaimaa Ismael, Dalia A. Hamza, Heba S. Farag, Karima Mogahed Fahim, Hanan S. Khalefa |
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| Rok vydání: | 2019 |
| Předmět: |
Serotype
Veterinary medicine food.ingredient General Veterinary 040301 veterinary sciences 0402 animal and dairy science 04 agricultural and veterinary sciences Biology medicine.disease_cause medicine.disease 040201 dairy & animal science Mastitis Milking 0403 veterinary science food medicine Agar Escherichia coli Somatic cell count Feces California mastitis test |
| Zdroj: | International Journal of Veterinary Science and Medicine. 7:61-70 |
| ISSN: | 2314-4599 |
| DOI: | 10.1080/23144599.2019.1691378 |
| Popis: | The study was conducted to estimate the prevalence of Escherichia coli (E. coli) in sub-clinically mastitic (SCM) animals, and in wild and migratory birds which may act as reservoir disseminating such pathogen. Farm hygiene, management and milking procedures were listed through a questionnaire. Thirty lactating cows and 15 lactating buffaloes from five small-scale dairy farms were randomly selected and screened for subclinical mastitis (SCM) using California Mastitis Test (CMT) and somatic cell count (SCC). In addition, 80 teat skin swabs, 5 drinking water samples and 38 wild and migratory bird faecal matter were also collected. All samples were processed for E. coli isolation by culturing on Levine's Eosin Methylene Blue (L-EMB) agar, followed by purification and biochemical identification. Positive samples were subjected to molecular identification and serotyping. In addition, the presence of extended-spectrum beta-lactamase (ESBL) and carbapenemase-producing E. coli have been reported by antimicrobial sensitivity testing. Escherichia coli were isolated from 7.7%, 50% and 50% of the positive CMT cows' quarters, cows' composite and buffaloes' composite milk samples, respectively. In addition, 14% of cows' teats, 20% of water samples, 70% of faecal matter from wild bird, and 33.3% of faecal matter from migratory waterfowls were carrying E. coli. Serotyping, antibiotic-resistant pattern and phylogenetic analysis have pointed the bearable implication of milking hygiene and wild birds in disseminating E. coli strains causing intramammary infections. |
| Databáze: | OpenAIRE |
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