| Autor: |
Li Yu, Shanshan Li, Haibo Liu, Guihua Tai, Jeff Iteku, Lin Sun, Yifa Zhou, Xu Zhang, Xiaoyu Liu |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Carbohydrate Polymers. 79:811-817 |
| ISSN: |
0144-8617 |
| DOI: |
10.1016/j.carbpol.2009.08.028 |
| Popis: |
Five rhamnogalacturonan I (RG-I) domains RG-I-1, RG-I-2, RG-I-3A, RG-I-3B and RG-I-4 were isolated from ginseng pectin by endo-polygalacturonase hydrolysis and a combination of ion-exchange and gel-permeation chromatography. The five domains all contain galacturonic acid, rhamnose, galactose and arabinose as main components and their rhamnose/galacturonic acid is from 0.26 to 0.64, among the range of RG-I. The molecular weights of RG-I-1 (5 kDa), RG-I-2 (4 kDa) and RG-I-3B (6 kDa) are smaller than those of RG-I-3A (45 kDa) and RG-I-4 (60 kDa). 13C NMR spectra of all domains showed RG-I features. RG-I-2 and RG-I-3B contained RG-I domains linked with highly methyl-esterified and acetylated homogalacturonan domains and the side chains probably belonging to type I and type II arabinogalactans, while RG-I-3A and RG-I-4 may have the side chains of type I arabinogalactans. 4-O-methyl-β- d -glucuronic acid residues were present at non-reducing terminals of RG-I-2. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect