Effect of irradiation spectral range on porphyrin—Protein complexes
| Autor: | Sergey A. Syrbu, Yury A. Gubarev, Elena S. Yurina, Natalya Sh. Lebedeva, Alexey Lyubimtsev |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology medicine.diagnostic_test General Chemical Engineering Proteolysis Tryptophan General Physics and Astronomy General Chemistry 010402 general chemistry medicine.disease 01 natural sciences Porphyrin Fluorescence 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry biology.protein medicine Biophysics Photosensitizer Irradiation Bovine serum albumin Cell damage |
| Zdroj: | Journal of Photochemistry and Photobiology A: Chemistry. 353:299-305 |
| ISSN: | 1010-6030 |
| DOI: | 10.1016/j.jphotochem.2017.11.037 |
| Popis: | The influence of irradiation on protein- porphyrin complexes at various spectral ranges was studied. Limited applicability of evaluating protein damage degree in terms of fluorescence quenching of the tryptophan protein residues was shown. Depending on the conditions of photoirradiation and the way of porphyrins interaction with bovine serum albumin (BSA), photoirradiation of the porphyrin-protein complexes may result in proteolysis or photolinking inside the protein globule, and in some cases, in destruction of the complex itself. P1 5-(4′-N-tertbutyloxycarbonylglicinaminophenyl)-10,15,20-triphenylporphine, unlike 5,10,15,20-tetraphenylporphin (TPP), has fixed localization in the protein due to “anchor” group providing the stability of the photoinduced transformations of a protein globule. For all porphyrin-protein systems studied, irradiation by light at the Soret band range for P1-protein systems leads to proteolysis of BSA globule, while the irradiation by light with the wavelength more than 500 nm, does not result in total protein damage, but causes oxidation of susceptible amino acid residues. The measurement of the hydrodynamic radii of a protein globule before and after irradiation provided objective information on the proceeding photoinduced processes (proteolysis and crosslinking) which can be used for predicting the mechanism of the cell damage (necrosis, autophagy, apoptosis). |
| Databáze: | OpenAIRE |
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