Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration
| Autor: | Bahareh Beiki, Ehsan Taghiabadi, Amir Bajouri, Nasser Aghdami |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Fetus integumentary system Chemistry Regeneration (biology) Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Membrane Tissue engineering 030220 oncology & carcinogenesis embryonic structures Skin substitutes Stem cell Bacterial outer membrane Wound healing |
| Zdroj: | Skin Stem Cells ISBN: 9781493988693 |
| DOI: | 10.1007/7651_2018_135 |
| Popis: | Various natural and synthetic biomaterials have been applied as skin substitutes for regenerating damaged skin. Here, we describe a straightforward method for fabrication of a tissue-engineered skin substitute by seeding human fetal fibroblasts on acellular human amniotic membrane (HAM). Fetal fibroblasts are achieved from the skin of normal and non-macerated fetus of 11-14 weeks old after spontaneous pregnancy termination. Acellular HAM is obtained by separation of the outer membrane of the chorion and removing its epithelial cells. |
| Databáze: | OpenAIRE |
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