Characteristics of aerobic methane-oxidising bacterial community at the sea-floor surface of the Nankai Trough
| Autor: | Masashi Hatamoto, Toshihiro Hoaki, Shinya Suzuki, Noriko Okita |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Ecology
biology Methane monooxygenase Sediment 04 agricultural and veterinary sciences 010501 environmental sciences Aquatic Science Particulates Oceanography 01 natural sciences Methane Petroleum seep chemistry.chemical_compound Nankai trough chemistry Environmental chemistry Anaerobic oxidation of methane 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Environmental science Ecology Evolution Behavior and Systematics Seabed 0105 earth and related environmental sciences |
| Zdroj: | Marine and Freshwater Research. 71:1252 |
| ISSN: | 1323-1650 |
| DOI: | 10.1071/mf19317 |
| Popis: | Methane hydrate is one of the new energy sources, but methane leakage could cause environmental issues such as marine ecosystem changes and global warming. The methane-oxidising bacterial (MOB) community could reflect the methane concentration, thus it may be an indicator of methane leakage. We obtained two sea-floor surface samples from a methane seep area and 12 samples from other general sea-floor areas of the Nankai Trough for the detection and phylogenetic analysis of the particulate methane monooxygenase (pmoA) gene. Using quantitative polymerase chain reaction analysis, the methane seep samples were found to have 106 copies of the pmoA gene per gram of sediment, whereas the general sea-floor area samples of the Nankai Trough contained 103–104 copies of the gene per gram of sediment. Phylogenetic analysis of the pmoA gene sequences showed that the sequences detected in the general and methane seep area samples differed significantly. Specifically, the pmof1–pmor primer pair could detect pmoA genes for the methane seep area, whereas pmoA gene from the general seafloor samples could be detected only using the A189–mb661 primer pair. The results of this study may facilitate the detection of unintended leakage of methane at methane hydrate production sites by monitoring MOB communities using pmoA-targeted phylogenetic analysis and quantification. |
| Databáze: | OpenAIRE |
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