Facile and sensitive detection of influenza viruses using SERS antibody probes
| Autor: | Eun Kyung Lim, Juyeon Jung, Jeong Moon, Jieun Sim, Bong Hyun Chung, Gayoung Eom, Bongsoo Kim, Taejoon Kang, So Yeon Yi, Ahreum Hwang, Jinyoung Jeong |
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| Rok vydání: | 2016 |
| Předmět: |
Detection limit
medicine.diagnostic_test biology Chemistry General Chemical Engineering 010401 analytical chemistry Influenza a 02 engineering and technology General Chemistry 021001 nanoscience & nanotechnology Highly selective 01 natural sciences Rapid detection Combinatorial chemistry Molecular biology 0104 chemical sciences Virus detection Antigen Immunoassay medicine biology.protein Antibody 0210 nano-technology |
| Zdroj: | RSC Advances. 6:84415-84419 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c6ra13966c |
| Popis: | It is highly important to identify influenza viruses rapidly and accurately for the prevention of future pandemic outbreaks. We developed an easy and sensitive influenza virus detection method using surface-enhanced Raman scattering (SERS) antibody probes. The SERS antibody probes can be prepared easily by mixing Au nanoparticles, gold binding peptide-protein G, and antibodies without complicated chemical or biological reactions. They also retain the optimal conformation for the antibody's interaction with Influenza A/CA/07/2009 (pH1N1), allowing us to detect pH1N1 sensitively and selectively. This method provides a detection limit of 4.1 × 103 TCID per mL and is highly selective for pH1N1. We anticipate that this method can be useful in a wide range of immunoassays. |
| Databáze: | OpenAIRE |
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