Spectroscopic Studies of Carotenoid-to-Bacteriochlorophyll Energy Transfer in LHRC Photosynthetic Complex from Roseiflexus castenholzii 1 Resubmitted to J Phys Chem B
| Autor: | Dariusz M. Niedzwiedzki, Harry A. Frank, Miriam M. Enriquez, Robert E. Blankenship, Amy M. LaFountain, Aaron M. Collins |
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| Rok vydání: | 2010 |
| Předmět: |
Photosynthetic reaction centre
chemistry.chemical_classification Absorption spectroscopy Chemistry organic chemicals food and beverages Chlorosome Photochemistry medicine.disease_cause Surfaces Coatings and Films chemistry.chemical_compound Ultrafast laser spectroscopy Materials Chemistry medicine Roseiflexus castenholzii Bacteriochlorophyll Physical and Theoretical Chemistry Spectroscopy Carotenoid |
| Zdroj: | The Journal of Physical Chemistry B. 114:8723-8734 |
| ISSN: | 1520-5207 1520-6106 |
| DOI: | 10.1021/jp1005764 |
| Popis: | Carotenoids present in the photosynthetic light-harvesting reaction center (LHRC) complex from chlorosome lacking filamentous anoxygenic phototroph, Roseiflexus castenholzii were purified and characterized for their photochemical properties. The LHRC from anaerobically grown cells contains five different carotenoids, methoxy-keto-myxocoxanthin, gamma-carotene, and its three derivatives, whereas the LHRC from aerobically grown cells contains only three carotenoid pigments with methoxy-keto-myxocoxanthin being the dominant one. The spectroscopic properties and dynamics of excited singlet states of the carotenoids were studied by steady-state absorption, fluorescence and ultrafast time-resolved optical spectroscopy in organic solvent and in the intact LHRC complex. Time-resolved transient absorption spectroscopy performed in the near-infrared (NIR) on purified carotenoids combined with steady-state absorption spectroscopy led to the precise determination of values of the energies of the S(1)(2(1)A(g)(-)) excited state. Global and single wavelength fitting of the ultrafast spectral and temporal data sets of the carotenoids in solvents and in the LHRC revealed the pathways of de-excitation of the carotenoid excited states. |
| Databáze: | OpenAIRE |
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