Bioassay standardization for water quality monitoring in Mexico

Autor: H. S. Hernández, Y. Pica‐Granados, G. D. Trujillo
Rok vydání: 2000
Předmět:
Zdroj: Environmental Toxicology. 15:322-330
ISSN: 1522-7278
1520-4081
DOI: 10.1002/1522-7278(2000)15:4<322::aid-tox8>3.0.co;2-f
Popis: In Mexico, bioassays for water toxicity testing can be useful tools for water quality control programs and environmental risk evaluation. However, since many bioassays have not been standardized, their application and acceptance in pollution monitoring and control sectors are limited. In an effort to overcome this limitation, the Mexican Institute of Water Technology (MIWT) agreed to participate in an international laboratory network (WaterTox), sponsored by the International Development Research Centre (IDRC). The Centre seeks to evaluate the analytical repeatability and reproducibility of six bioassays (Allium, Hydra, Daphnia magna, and Panagrellus assays; lettuce seed germination and Fluctuation tests) and to determine their sensitivity patterns and the manner in which they are complementary or redundant in a core test battery. In addition, the Mexican team added the Vibrio fischeri Microtox toxicity test to the evaluation. A series of 24 blind samples were analyzed over a 1-year period. Results indicated that precision, or repeatability, was acceptable for most bioassays. For blind sample replicates, the overall coefficient of variation was less than 22%, except for the Muta-ChromoPlate test (60%) and the nematode assay (greater than 100%). While most toxic samples were detected by D. magna and Hydra attenuata assays, those that produced little effect on these two organisms affected lettuce and onion root elongation. The complementary nature of these tests provides a wide spectrum of toxicant detection, in contrast to the Microtox test which showed a more limited and redundant sensitivity pattern when compared against a battery comprised of the four inexpensive bioassays. © 2000 John Wiley & Sons, Inc. Environ Toxicol 15: 322–330, 2000
Databáze: OpenAIRE
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