Influence of erythrocyte shape on the rate of Ca2+-induced scrambling of phosphatidylserine

Autor:	Paul Comfurius, Jef L. N. Wolfs, Robert F. A. Zwaal, Edouard M. Bevers
Rok vydání:	2003
Předmět:	Calcium metabolism Phospholipid scramblase Echinocyte chemistry.chemical_element Cell Biology Phosphatidylserine Calcium Microvesicles chemistry.chemical_compound chemistry Biochemistry Prothrombinase Ionomycin Biophysics Molecular Biology
Zdroj:	Molecular Membrane Biology. 20:83-91
ISSN:	1464-5203 0968-7688
DOI:	10.1080/0968768031000064444
Popis:	Membrane-perturbing agents that cause transformation of biconcave erythrocytes into echinocytes or stomatocytes were used to investigate the influence of erythrocyte shape on the rate of Ca(2+)-induced scrambling of phospholipids. Erythrocytes were treated with a variety of lipid-soluble compounds to induce these shape changes, followed by incubation with calcium and ionomycin to activate lipid scramblase. Prothrombinase activity of the cells was used to monitor the rate of surface exposure of phosphatidylserine, which is taken as a measure of scramblase activity. Echinocytes show an enhanced rate of scrambling, whereas stomatocytes show a reduced rate, relative to normocytes. This phenomenon appears to correlate with enhanced and diminished micro-exovesicle shedding from echinocytes and stomatocytes, respectively. It is concluded that the rate of calcium-induced phosphatidylserine exposure (rate of lipid scrambling) in erythrocytes depends for a considerable part on the cells' ability to form microvesicles.
Databáze:	OpenAIRE
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