Microinjection and Micropipette-Controlled Phagocytosis Methods for Neutrophils
| Autor: | Jennie S. Campbell, Maurice Bartlett Hallett, Iraj Laffafian, Sharon Dewitt |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell specific Chemistry Phagocytosis media_common.quotation_subject Cell Pipette 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure 030220 oncology & carcinogenesis medicine Biophysics Internalization Microinjection media_common |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071601532 |
| DOI: | 10.1007/978-1-0716-0154-9_9 |
| Popis: | The ability to microinject substances into the cytosol of living neutrophils opens the possibility of manipulating the chemistry within the cell and also of monitoring changes using indicators which otherwise cannot be introduced into the cell. However, neutrophils cannot be microinjected by the conventional glass pipette insertion method. Here we outline two techniques which work well with neutrophils, namely, SLAM (Simple Lipid-Assisted Microinjection) and electromicroinjection. As these methods utilize micropipettes, we also include a simple method which uses a micropipette to deliver a phagocytic stimulus to a specific cell at a defined time, enable detailed study of the phagocytic process from particle contact to particle internalization. |
| Databáze: | OpenAIRE |
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