Endurance Capacity of Muscle‐specific Branched‐chain alpha‐Ketoacid Dehydrogenase (BCKDH) Kinase‐deficient Mice

Autor: Takanari Toyoda, Yoshiharu Shimomura, Minjun Xu, Rina Kato, Takuya Ishikawa, Jussiaea Valente Bariuan, Takashi Morioka, Yukako Morishita, Miki Ota, Yusuke Kondo, Yoshihiro Kadota, Yasuyuki Kitaura, Hongmin Zhen
Rok vydání: 2015
Předmět:
Zdroj: The FASEB Journal. 29
ISSN: 1530-6860
0892-6638
Popis: The main catabolic pathway of branched-chain amino acids (BCAAs: leucine, isoleucine, and valine) is located in mitochondria. The rate-limiting step of the catabolism in muscle tissues is catalyzed by BCKDH complex, which is located at the second step in the pathway. The activity of BCKDH complex is regulated by covalent modification, and BCKDH kinase (BDK) is responsible for inactivation of the complex by phosphorylation. In the present study, we produced muscle-specific BDK knockout (BDK-mKO) mice. The muscle BCAA concentrations were less than half in BDK-mKO mice than in control mice. We assessed the endurance capacity of BDK-mKO mice and control mice at 12 weeks of age (under untrained conditions) and at 21 weeks of age after 9 weeks training by measuring the distance of running to exhaustion using treadmill with 10% grade. The running speed in the test was initial 15 m/min for 4 min and then increased at the rate of 1 m/min every 4 min. The training was performed 5 days/week by running for 60 min/day...
Databáze: OpenAIRE
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