| Popis: |
Results from interactions of the 11-cis and 9-cis isomers of eleven fluorinated phenylretinal analogs, prepared from fluorinated benzaldehydes, with bovine opsin have been examined. Five of these (2′,6′-bis-CF3, 2′,4′,6′-tris-CF3, 2′-CF3-6′-F, 2′-CF3-7-methyl and 2′-CF3,6′-F,8-F) formed pigments in moderate to high yields, thus allowing recording of their 19F-NMR spectra which revealed inhibited conformational equilibration when protein bound. Possible causes for binding selectivity and fluorine chemical shifts are discussed. |
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