Combined thermal field-flow fractionation–capillary electrophoresis

Autor:	S. N. Semenov
Rok vydání:	1998
Předmět:	Temperature gradient Viscosity Field flow fractionation Capillary electrophoresis Capillary action Chemistry Analytical chemistry Joule heating Thermophoresis Analytical Chemistry Separation process
Zdroj:	Analytical Communications. 35:229-233
ISSN:	1364-5536 1359-7337
DOI:	10.1039/a803366h
Popis:	The possibility of using the transverse thermophoresis of analytes in the capillary used in capillary electrophoresis (CE) to control the separation process was considered, thus decreasing the spreading of the thermal peak and providing new separation parameters in CE. The Joule heating in non-aqueous buffers in the CE capillary can provide transverse temperature gradients comparable to temperature gradients in conventional equipment for thermal field-flow fractionation (ThFFF). It is proposed to use the non-uniform velocity profile of analytes caused by transverse temperature gradient and the temperature dependence of the buffer viscosity for the FFF-like separation of analytes in addition to CE separation. The expressions for the peak parameters are derived, taking into account the non-uniform transverse analyte distribution due to the thermophoresis, and the possibilities based on FFF–CE principles are discussed. As possible objects of this hyphenated technique, macromolecules and particles are considered.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bc786ecc60af3adcbf63002fdca55d09 https://doi.org/10.1039/a803366h Zobrazit plný text záznamu