Popis: |
A new fluorescence band at (400÷430) nm for many carboxylic and amino acids is reported. An excitation band with a maximum around 330 nm is always observed, while further bands can be present at lower wave-lengths. The dependence of the fluorescence intensity has been studied as a function of ionic strength, temperature, concentration andpH. ThepH of the solution strongly affects the fluorescence yield which reaches its maximum atpH lower than 2. This suggests the main role of undissociated carboxylic group as responsible for the observed fluorescence. Finally, an interpretation of the experiments is suggested on the basis of the theoretical analysis available up to now. |