Visual evoked potential in rabbits’ eyes with subretinal implantation by vitrectomy of Okayama University-type retinal prosthesis (OURePTM)
| Autor: | Masao Oguchi, Mamoru Tanaka, Daisuke Ido, Shigiko Takei, Toshinori Furukawa, Tetsuya Uchida, Koichiro Yamashita, Toshihiko Matsuo |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
Retina Materials science General Veterinary medicine.medical_treatment 0206 medical engineering Retinal detachment Retinal Vitrectomy 02 engineering and technology medicine.disease 020601 biomedical engineering 03 medical and health sciences chemistry.chemical_compound Light intensity 0302 clinical medicine medicine.anatomical_structure chemistry Ophthalmology Retinal Prosthesis 030221 ophthalmology & optometry medicine Evoked potential Laser coagulation |
| Zdroj: | Journal of Veterinary Medical Science. 80:247-259 |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | Okayama University-type retinal prosthesis (OURePTM) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to test surgical feasibility for subretinal film implantation and to examine functional durability of films in subretinal space. Dye-coupled films were implanted subretinally by vitrectomy in the right eye of normal white rabbits: 8 rabbits for 1 month and 8 rabbits for 6 months. The implanted films were removed by vitrectomy in 4 of these 8 rabbits in 1-month or 6-month implantation group. The films were also implanted in 4 rhodopsin-transgenic retinal dystrophic rabbits. Visual evoked potential was measured before film implantation as well as 1 or 6 months after film implantation, or 1 month after film removal. The films were successfully implanted in subretinal space of retinal detachment induced by subretinal fluid injection with a 38G polyimide tip. The retina was reattached by fluid-air exchange in vitreous cavity, retinal laser coagulation, and silicone oil injection. The ratios of P2 amplitudes of visual evoked potential in the implanted right eye over control left eye did not show significant changes between pre-implantation and post-implantation or post-removal (paired t-test). In Kelvin probe measurements, 4 pieces each of removed films which were implanted for 1 or 6 months showed proportional increase of surface electric potential in response to increasing light intensity. The film implantation was safe and implanted films were capable of responding to light. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|