The Influence of Bovine Serum Albumin Modified Titanium Dioxide Nanoparticles on Myoblast Cytotoxicity

Autor: Ajit Kumar, Wendy N. Phoswa, Irene Mackraj, Kogi Moodley, Preenan Pillay
Rok vydání: 2020
Předmět:
Zdroj: Pharmaceutical Chemistry Journal. 54:883-890
ISSN: 1573-9031
0091-150X
DOI: 10.1007/s11094-020-02292-x
Popis: Titanium dioxide nanoparticles (TiO2NPs) are utilised as a key constituent in many biopharmaceuticals and food products. This is mainly attributed to their favorable chemical properties which enhance their biocompatibility. However, alteration of the physicochemical properties in various matrices remains unexplored. The present study aims to determine if TiO2NPs modified by bovine serum albumin (BSA) have the ability to influence cytotoxicity in C2C12 myoblast cells. The physicochemical properties of TiO2NPs were altered using BSA in a concentration range of 0.3 – 1.5 mg/mL and characterised using nanoparticle (NP) tracking analysis and transmission electron microscopy. Results show that cytotoxicity is enhanced at a BSA concentration of 0.8 mg/mL, which agrees with reduced particle agglomeration and favorable zeta potential. This proves that the state of TiO2 NPs agglomeration directly affects cytotoxicity which can be due to internalization of the smaller nanoparticle (NP) aggregates. These findings suggest that in vitro cytotoxicity assays incorporate the physicochemical characteristics of NPs in relation to the specific cell model used and colloidal state of the substance being tested.
Databáze: OpenAIRE
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