A D-vitamin metabolizmusa humán hepatocellularis carcinomában és az azt körülvevő tumormentes májszövetben

Autor:	Áron Somorácz, András Kiss, Aron Lazary, Anna Korompay, Ferenc Szalay, János P. Kósa, D Németh, Benedek Gyöngyösi, Péter Kupcsulik, Bernadett Balla, É. Horváth, Hasan Jozilan, Katalin Borka, Zsuzsa Schaff, P. Lakatos
Rok vydání:	2016
Předmět:	0301 basic medicine 03 medical and health sciences 030104 developmental biology 0302 clinical medicine business.industry 030220 oncology & carcinogenesis Medicine General Medicine business Molecular biology
Zdroj:	Orvosi Hetilap. 157:1910-1918
ISSN:	1788-6120 0030-6002
DOI:	10.1556/650.2016.30592
Popis:	Introduction: 1,25-Dihydroxy vitamin D3 mediates antitumor effects in hepatocellular carcinoma. Aim: We examined mRNA and protein expression differences in 1,25-Dihydroxy vitamin D3-inactivating CYP24A1, mRNA of activating CYP27B1 enzymes, and that of VDR between human hepatocellular carcinoma and surrounding non-tumorous liver. Methods: Snap-frozen tissues from 13 patients were studied for mRNA and protein expression of CYP24A1. Paraffin-embedded tissues from 36 patients were used to study mRNA of VDR and CYP27B1. mRNA expression was measured by RT-PCR, CYP24A1 protein was detected by immunohistochemistry. Results: Expression of VDR and CYP27B1 was significantly lower in hepatocellular carcinoma compared with non-tumorous liver (p3, providing an escape mechanism from the anti-tumor effect. Orv. Hetil., 2016, 157(48), 1910–1918.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bb89c8c79fc42f4b1f9e6fe55852436b https://doi.org/10.1556/650.2016.30592 Zobrazit plný text záznamu