A new aspect of in vitro antimicrobial leukocyte- and platelet-rich plasma activity based on flow cytometry assessment
| Autor: | Agata Cieślik-Bielecka, Paweł Reichert, Rafał Skowroński, Aleksandra Królikowska, Tomasz Bielecki |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.diagnostic_test Chemistry animal diseases Hematology General Medicine 030204 cardiovascular system & hematology Antimicrobial medicine.disease_cause In vitro nervous system diseases Microbiology Flow cytometry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Thrombin Staphylococcus aureus Platelet-rich plasma medicine Platelet Escherichia coli medicine.drug |
| Zdroj: | Platelets. 30:728-736 |
| ISSN: | 1369-1635 0953-7104 |
| DOI: | 10.1080/09537104.2018.1513472 |
| Popis: | The current literature suggests that the antibacterial effect of leukocyte- and platelet-rich plasma (L-PRP) is directly related to platelet and leukocyte concentrations. The aim of this study was twofold: first, to evaluate the antimicrobial effect of L-PRP against selected bacterial strains in vitro, and second, to correlate this effect with leukocyte and platelet content in the final concentration. Blood was collected from 20 healthy males, and L-PRP, acellular plasma (AP), and autologous thrombin were consecutively prepared. Flow cytometry analysis of the blood, L-PRP, and AP was performed. The L-PRP gel, liquid L-PRP, and thrombin samples were tested in vitro for their antibacterial properties against seven selected bacterial strains using the Kirby-Bauer disk-diffusion method. There was notable antimicrobial activity against selected bacterial strains. No statistically significant correlations between antimicrobial activities and the platelet concentration in L-PRP were observed. Statistically significant positive correlations between selected leukocyte subtypes and antimicrobial activity were noted. A negative correlation was found between elevated monocyte count and antimicrobial activity of L-PRP against one bacterial strain studied. L-PRP possesses antimicrobial activity and can be potentially useful in the fight against certain postoperative infections. The bactericidal effect of L-PRP is caused by leukocytes, and there exists a relationship among selected leukocyte subtypes and L-PRP antimicrobial activity. |
| Databáze: | OpenAIRE |
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