Mechanism of primisulfuron resistance in a shattercane (Sorghum bicolor) biotype
| Autor: | Alex Martin, Scott J. Nissen, Fred W. Roeth, Daniel D. Anderson |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
0106 biological sciences
Acetolactate synthase biology Chemistry medicine.drug_class Sorghum bicolor Chromosomal translocation 04 agricultural and veterinary sciences Plant Science Sorghum biology.organism_classification 01 natural sciences Sulfonylurea 010602 entomology Animal science Shoot 040103 agronomy & agriculture biology.protein medicine 0401 agriculture forestry and fisheries Previously treated Agronomy and Crop Science After treatment |
| Zdroj: | Weed Science. 46:158-162 |
| ISSN: | 1550-2759 0043-1745 |
| DOI: | 10.1017/s0043174500090354 |
| Popis: | A shattercane biotype resistant to primisulfuron was identified during greenhouse evaluations of seed obtained from southeastern and south-central Nebraska fields previously treated with primisulfuron or nicosulfuron a minimum of 3 consecutive yr. Absorption, translocation, metabolism, and acetolactate synthase (ALS) assay experiments were conducted to determine resistance mechanism(s) by comparing ALS-susceptible forage sorghum (ROX) to resistant shattercane (RS). The ROX had 10 and 12% greater14C absorption than RS 24 and 96 hours after treatment (HAT), respectively. Absorption of14C increased over time for both ROX and RS, while14C translocation from the treated leaf to the shoots and roots was similar for ROX and RS. Unmetabolized primisulfuron accounted for > 80% of the radioactivity recovered 24 h after application for both ROX and RS. The ROX and RS had similar ALS-specific activities and total protein concentrations.Kmvalues for RS and ROX were 10.4 and 5.8 mM pyruvate, respectively. The ALS isolated from RS was less sensitive than ROX to inhibition by primisulfuron. The I50values for RS and ROX were 231 and 0.025 μM primisulfuron, respectively. The mechanism of primisulfuron resistance in this RS biotype is an altered ALS with decreased sensitivity to primisulfuron. |
| Databáze: | OpenAIRE |
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