| Popis: |
Purpose Prolonged lipid malabsorption occurs after small bowel resection (SBR). We aimed to clarify the underlying mechanism using a mouse model. Methods SBR mice were created by resecting 50% of the proximal intestine. In sham mice, the intestine was transected and re-anastomosed. Intravenous nutrition was not initiated and mice were fed a liquid elemental diet followed by a solid standard rodent diet for one week each. Animals were sacrificed on postoperative day (POD)7 or POD14, and the following data were analyzed: body weight, caloric intake, histopathological findings, and the mRNA expression of genes related to intestinal lipid absorption in the remnant intestines (jejunum and ileum). Results SBR group showed significantly delayed body weight recovery, despite comparable caloric intake. A histopathological examination revealed increased villus height and deepening crypts in the remnant jejunum and ileum as “morphological adaptation” after SBR. Importantly, huge differences in the mRNA expression of genes related to intestinal lipid absorption were observed between the remnant jejunum and ileum, and above differences were only slight changed after SBR on both POD7 and POD14. Conclusions Insufficient compensation of the gene expression related to intestinal lipid absorption might be the cause of prolonged lipid malabsorption after SBR. |
