Relevance of Protein Content within the Renal Scaffold for Kidney Bioengineering and Regeneration
| Autor: | Fatima Guerrero, Sagrario Cañadillas, Andrés Carmona, Concha Herrera, Rodolfo Crespo, Rosa Ortega, Pedro Aljama |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Kidney Scaffold Pathology medicine.medical_specialty Decellularization business.industry medicine.medical_treatment 030230 surgery Bioinformatics medicine.disease Transplantation 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure medicine Hemodialysis business Kidney transplantation Homing (hematopoietic) Kidney disease |
| Zdroj: | Journal of Biomedical Science and Engineering. 10:509-526 |
| ISSN: | 1937-688X 1937-6871 |
| DOI: | 10.4236/jbise.2017.1011039 |
| Popis: | Chronic kidney disease is currently a major public health problem around the world. Although hemodialysis increases survival of patients with end-stage renal disease, kidney transplantation remains the only potentially curative treatment. However, transplantation as a therapeutic option is limited by availability of suitable donor organs. This situation highlights the urgent need to find new and potentially inexhaustible sources of transplantable organs. Perfusion decellularizarion of whole organs is a novel approach to organ engineering and regeneration. In the present research, we used a continuous perfusion decellularization protocol to eliminate cellular componet of kidney and evaluated residual scaffold components after decellularizarion process by proteomics analysis. Our proteomic data show that this protocol results in incomplete removal of cellular proteins. However, unlike other authors, we assume that proteins retained within decellularized kidney scaffold could be the basis for specific homing and celular differentation in the recellularization process. |
| Databáze: | OpenAIRE |
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