| Autor: |
A. R. Prosser, Willard D. Hubbard, Alan J. Sheppard |
| Rok vydání: |
1977 |
| Předmět: |
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| Zdroj: |
Journal of AOAC INTERNATIONAL. 60:895-898 |
| ISSN: |
0004-5756 |
| DOI: |
10.1093/jaoac/60.4.895 |
| Popis: |
The Canadian FA-59 enzymatic method for determining cis,cis-methylene interrupted polyunsaturated fatty acids has been modified to meet the needs of the cholesterol and fatty acid regulations of the U.S. Food and Drug Administration. A centrifugation step has been introduced to remove palmitate and stearate interference with absorbance readings for samples containing low amounts of cis,cis-methylene interrupted polyunsaturated fatty acids. The data are calculated by using a standard curve and an equation which yields g trilinolein/100 g product rather than % linoleic acid in a given weight of sample. The standard curve was linear for 1–12 μg trilinolein. Recovery of added trilinolein was 99.5%, with 4% coefficient of variation. The modified procedure was successfully applied to a wide variety of foodstuffs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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