Determination of Formate Using Immobilized Formate Dehydrogenase in a Flow System and Its Application to Analyze the Formate Content of Foodstuffs

Autor:	Hisakazu Mori, Ryoko Ohmori
Rok vydání:	2008
Předmět:	Peak area Flow injection analysis chemistry.chemical_compound Flow system Chromatography Immobilized enzyme Calibration curve Chemistry Health Toxicology and Mutagenesis education Formate Toxicology Formate dehydrogenase
Zdroj:	Journal of Health Science. 54:212-215
ISSN:	1347-5207 1344-9702
Popis:	The quantity of formate was determined using apparatus comprised of a reactor with immobilized formate dehydrogenase (FDH) in the flow line. NADH formed by an enzymatic reaction was fluorometrically detected. The optimal concentration of NAD + in the carrier was determined. The maximum peak area due to NADH was observed at pH 7.0 when the pH of the carrier consisting of piperazine1,4-bis(2-ethanesulfonic acid) (PIPES) buffer ranged from 6.0 to 8.0. Various buffer types were also examined as carrier media at pH 7.0 and PIPES buffer showed the maximum peak area. When the carrier composed of PIPES buffer (0.1M, pH 7.0) was used, the calibration curve for formate was linear in the range of 0.5‐50µ M( r = 1.000). Relative standard deviations of the peak area at 1µ Ma nd 10µM were 2.6% (n = 7) and 1.6% (n = 7), respectively. This method was applied to the analysis of formate in foodstuffs, and formate content determined by this method agreed with that determined by a commercially available test-kit.
Databáze:	OpenAIRE
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