Augmentation of degradation prospects of dioxygenases from the crude extract of an efficient bacterial strain, using pyrene as sole carbon source
| Autor: | Bineypreet Kaur, Bulbul Gupta, Jaspreet Kaur |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
010302 applied physics
chemistry.chemical_classification Catechol Microorganism 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences chemistry.chemical_compound Bioremediation Enzyme chemistry Dioxygenase 0103 physical sciences Degradation (geology) Pyrene Organic chemistry 0210 nano-technology Carcinogen |
| Zdroj: | Materials Today: Proceedings. 28:1690-1694 |
| ISSN: | 2214-7853 |
| DOI: | 10.1016/j.matpr.2020.05.129 |
| Popis: | In recent times, microorganisms have been screened, contemplating their potential to produce various enzymes of domestic as well as industrial importance. These enzymes possess substantial propensity for the bioremediation of carcinogenic and toxic aromatic contaminants. Catechol 1,2-dioxygenase as well as catechol 2,3-dioxygenase, familiar dioxygenases, are involved in specialized pathways known as meta- or ortho- cleavage pathway for the degradation of polycyclic aromatic hydrocarbons (PAHs). This report emphasizes characterization of one of the microbial strains, BJ5, adapted for higher production of catechol 1,2-dioxygenase and catechol 2,3-dioxygenase, using pyrene as the sole carbon source. The functionality of these enzymes is critically regulated by factors such as environmental state, pH, temperature and metal ions. Hence, in this study, specific activities, kinetic constants, pH and temperature optima were determined. These dioxygenase enzymes from the crude extract of BJ5 strain can act as alternative green catalysts for bioremediation resolution. |
| Databáze: | OpenAIRE |
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