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SummaryShoot explants of Prunus cerasifera cv Thundercloud and Spiraea × bumalda cv Froebelii were cultured on Linsmaier and Skoog (1965) medium with the addition of benzy- ladenine. Explant length, axillary bud number, presence or absence of the shoot apex and derivation of the explant were varied. Shoot proliferation was enhanced by reduction of axillary bud number, and at low BA concentrations by removal of the meristem tip. Large explants (10–20 mm) produced a greater number of shoots than small explants (5–10 mm), and shoot proliferation was greatest in explants derived from the top of the canopy of the parent plant. These results suggest that explant variability due to origin may account for the differences in morphogenic response frequently observed during micropropagation. |
