Effects of age on the immune response in baboons to the Y. pestis F1 vaccine antigen. (166.24)
| Autor: | Caroline Bonnel, Lourdes Arteaga-Cortes, Yasmin Ench, M. Leland, Peter Dube, Ellen Kraig |
|---|---|
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 188:166.24-166.24 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.188.supp.166.24 |
| Popis: | The ability to mount effective immune responses declines with age, which correlates clinically with decreased vaccine efficacy and increased susceptibility to infection and cancer. As a result, new vaccines should be tested for efficacy in older individuals. Towards this end, young (5-6 years of age) and old (17-22 years) baboons were immunized with the LcrV candidate vaccine antigen from Yersinia pestis. Surprisingly, older baboons had a strong humoral response [JI 181:109] suggesting either that the baboon immune system is less affected by aging or that LcrV acts like a recall antigen. Thus, a second Y. pestis vaccine antigen, the F1 protein, was tested; F1 is unique to this bacterial species and would be seen as a “new” antigen. The antibody response to F1 did decline with age, but the T cell response did not. Thus, an analysis of the effects of aging on T cell fine specificity was undertaken. T cell proliferation and IFN-γ ELISpots were used to map which of 32 overlapping synthetic F1 peptides stimulated T cells from the immunized baboons. A Th1 response was observed for a few individual animals in response to whole both F1 antigen and specific peptides. ELISpots specific for IL-4 and IL-5 cytokines are currently being used to follow Th2 cells specific for F1. Future efforts will focus on generating F1-specific T cell clones using herpesvirus papio transformed B cell lines as antigen presenting cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|