Counter-current chromatography using supercritical fluid carbon dioxide as mobile phase
| Autor: | Yi Hsing Chen, Shu En Li, Tiing Yu, H. Paul Wang |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Chromatography
Organic Chemistry Analytical chemistry Supercritical fluid extraction Separator (oil production) General Medicine Biochemistry Supercritical fluid Analytical Chemistry Physics::Fluid Dynamics chemistry.chemical_compound Countercurrent chromatography chemistry Carbon dioxide Benzophenone Supercritical fluid chromatography Methanol |
| Zdroj: | Journal of Chromatography A. 724:91-96 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/0021-9673(95)00925-6 |
| Popis: | Supercritical fluid carbon dioxide was employed in solar eccentric counter-current chromatography (SECCC) to replace the liquid mobile phase in the traditional arrangement. The fundamental difference from the conventional liquid-liquid system is the requirement for high-pressure operation. The experimental set-up is presented to illustrate the necessary modifications that accomodate operation with a supercritical fluid. Studies on the stationary phase retention due to various experimental conditions are discussed. A mixture of acetophenone and benzophenone was chromatographed using mixtures of water and methanol as the stationary solvent system to demonstrate the feasibility of this technique. At present the efficiency of the separation is not satisfactory owing to the phase separator inserted in the system to remove liquid droplets which contributed to considerable peak broadening. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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