Estimation of the Mean Degree of Polymerization of Condensed Tannins from the Kernel and Shell of Carya illinoinensis by HPLC/MS and Spectrophotometric Methods
| Autor: | M. A. Lerma-Herrera, Emilio Alvarez-Parrilla, José A. Núñez-Gastélum, Juan A. Ascacio-Valdés, L.A. de la Rosa, Joaquín Rodrigo-García, Ángel G. Díaz-Sánchez, Cristóbal N. Aguilar |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification 030109 nutrition & dietetics Chromatography Chemistry Depolymerization Extraction (chemistry) Size-exclusion chromatography 04 agricultural and veterinary sciences Fractionation Degree of polymerization 040401 food science Applied Microbiology and Biotechnology High-performance liquid chromatography Analytical Chemistry body regions 03 medical and health sciences 0404 agricultural biotechnology Proanthocyanidin parasitic diseases Tannin Safety Risk Reliability and Quality Safety Research Food Science |
| Zdroj: | Food Analytical Methods. 10:3023-3031 |
| ISSN: | 1936-976X 1936-9751 |
| Popis: | Rapid estimation of the degree of polymerization (DP) of pure tannins or mean degree of polymerization (mDP) of tannin samples is an analytical challenge and a necessity to better understand their bioactivity. In the present paper, four spectrophotometric analyses and normal phase HPLC/MS were performed to estimate mDP of tannin fractions from pecan nut (Carya illinoinesis) kernels and shells. Tannins were obtained by acetone extraction of phenolic compounds followed by size exclusion chromatography fractionation. Four final fractions were obtained, and their mDP was estimated by spectrophotometric and HPLC/MS analysis and results compared among the different methods. All assays showed good reproducibility. The combination of butanol–HCl depolymerization reaction with vanillin-acetic acid assay yielded mDP values equal to those estimated by HPLC/MS (alpha = 5%), proving this method both suitable and reliable for rapid mDP estimation of tannins in samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink