Rapid Micropropagation of Edible BambooDendrocalamus asper
| Autor: | Sarita Arya, Inder Dev Arya, Richa Satsangi |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Bamboo
Renewable Energy Sustainability and the Environment fungi Geography Planning and Development food and beverages Forestry Management Monitoring Policy and Law Biology biology.organism_classification Tissue culture Micropropagation Axillary bud Botany Shoot Dendrocalamus asper Subculture (biology) Food Science Explant culture |
| Zdroj: | Journal of Sustainable Forestry. 14:103-114 |
| ISSN: | 1540-756X 1054-9811 |
| DOI: | 10.1300/j091v14n02_06 |
| Popis: | Micropropagation protocols for Dendrocalamus asper using nodal shoots and seeds culture are described. Multiple shoots were induced through forced axillary branching. Ninety-five percent of the nodal shoot explants taken from juvenile primary and lateral branches, produced multiple shoots through axillary buds activation within 2 to 8 weeks on Murashige & Skoog's (MS) medium supplemented with 0.1-15 mg/l benzyladenine (BA). The cultured seeds also produced multiple shoots (1-20) within 6 weeks on this medium. The multiple shoot differentiation was influenced by the concentration of BA in the medium. The in vitro generated shoots were excised and subculture on MS + 3.0 mg/l BAP for further shoot multiplication. Fifteen to 20 fold rate of shoot multiplication was achieved by regular subculturing. These shoots were multiplied for more than 3 years without loss of vigor. Ninety-five percent of the shoots were rooted, when propagules (each consisting of cluster of 3 shoots) were transferred on to MS m... |
| Databáze: | OpenAIRE |
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