Production of ML-236B, and Inhibitor of 3-Hydroxy-3-methylglutaryl CoA Reductase, byPenicillium citrinum:Improvements of Strain and Culture Conditions
| Autor: | Hiroji Yoshikawa, Tetsuya Shioiri, Masatoshi Arai, Masahiko Hosobuchi, Jo Ohyama, Seigo Iwado |
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| Rok vydání: | 1993 |
| Předmět: |
Strain (chemistry)
Organic Chemistry Substrate (chemistry) General Medicine Maltose Biology Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry chemistry.chemical_compound Polypropylene glycol chemistry Pulmonary surfactant Glycerol Fermentation Penicillium citrinum Food science Molecular Biology Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 57:1414-1419 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.57.1414 |
| Popis: | In order to increase the yield of ML-236B, an intermediate for pravastatin Na (an inhibitor of cholesterol synthesis) production, the improvements of an ML-236B producing strain of Penicillium citrinum, the medium composition, and the culture conditions were studied. A mutant strain S-5808, which produces ML-236B 20 times more than the original strain does in flask culture, was isolated. As the cells of S-5808 required a large amount of the carbon source substrate to produce a lot of ML-236B, a fed-batch culture method was developed. The continuous feeding of carbon source (glycerol or mixture of glycerol and maltose) was very effective for the increase of ML-236B production.ML-236B in the culture broth became oily with lowering of pH during cultivation, adhered to the cell surface, decreased the carbon source consumption and finally stopped the ML-236B production at a late period of fermentation. The addition of a polypropylene glycol type surfactant enhanced the ML-236B productivity. The addition of the... |
| Databáze: | OpenAIRE |
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