Lysozyme inactivation by inert gas bubbling: kinetics in a bubble column reactor

Autor:	Alain Gaunand, Brigitte Lindet, Henri Planche, Sophie Colombié, Pierre Monsan, Mylène Caussette
Rok vydání:	1999
Předmět:	Aqueous solution Chromatography Kinetics chemistry.chemical_element Bioengineering Applied Microbiology and Biotechnology Biochemistry Nitrogen Volumetric flow rate chemistry.chemical_compound Adsorption Chemical engineering chemistry Lysozyme Inert gas Biotechnology Bubble column reactor
Zdroj:	Enzyme and Microbial Technology. 24:412-418
ISSN:	0141-0229
Popis:	The article focuses on lysozyme inactivation at gas–liquid interfaces. Bubbling nitrogen in a lysozyme aqueous solution strongly enhances enzyme inactivation. At a 150-ml min−1 flow rate, the half-life of lysozyme is 12 min while in the same conditions without bubbling no activity loss is observed after 8 h. Reported effects vary linearly with the interfacial area which shows that the inactivation is an interfacial mechanism. The inactivation induced by nitrogen bubbling strongly depends on temperature and pH acting on the adsorption process. This study points out on the one hand the importance of controlling gas–liquid interfaces in bioreactors and on the other hand the potentialities of an inactivation process using gas–liquid interfaces.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b23097b5ffff77f63e4b631c871b54ce https://doi.org/10.1016/s0141-0229(98)00151-3 Zobrazit plný text záznamu Full Text from ScienceDirect