| Autor: |
Brian J. Burrows, Alan P. Kypson, Maria C. Collins, Barbara J. Muller-Borer, Joel L. Moore |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
The Open Tissue Engineering and Regenerative Medicine Journal. 5:17-24 |
| ISSN: |
1875-0435 |
| DOI: |
10.2174/1875043501205010017 |
| Popis: |
Background: Human mesenchymal stem cells (hMSCs) show potential for therapeutic cellular cardiomyo- plasty. However, a range of delivery methods, including direct intramyocardial injection, have resulted in poor engraftment in vivo. We used the in vivo rat heart model to study hMSC engraftment and retention in a normal beating heart. Materials and Methods: HMSCs transfected with green fluorescent protein were injected into the left ventricle (LV) of immunocompetent rats. Hearts were cryopreserved 30 minutes (Group A), 24 hours (Group B), and 5 days (Group C) post hMSC delivery. HMSC retention was estimated using confocal fluorescence microscopy and immunohistochemistry. Measured values were compared to projected GFP-positive cellular volumes. Immunohistochemical analyses probed for the presence of human cells with human prolyl 4-hydroxylase beta (p4hβ) and an immune response with murine monocyte/macrophage antigen (CD68). Results: HMSC retention decreased significantly from 30 minutes to 5 days (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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