Detection of antibodies to HLA1 antigens by enzyme immunoassay
| Autor: | F. S. Baranova, A. Yu. Baryshnikov, T. N. Palkina, S. D. Borozdenkova |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.diagnostic_test
biology medicine.drug_class Chemistry Lymphocyte General Medicine Human leukocyte antigen Monoclonal antibody Molecular biology General Biochemistry Genetics and Molecular Biology Histocompatibility medicine.anatomical_structure Antigen Immunoassay medicine biology.protein Antibody Pan-T antigens |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 120:954-956 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/bf02445028 |
| Popis: | A modification of the MAILA (monoclonal antibody specific immobilization of lymphocyte antigens) method has been developed for the detection of antibodies to class 1 histocompatibility antigens. Russian biotin-treated monoclonal antibodies IKO-53 (Medbiospektr, Moscow) were used. In a complex with monoclonal antibodies, lymphocyte HLA antigen was found to retain its antigenic properties when stored for a long time. High specificity and sensitivity of the method were demonstrated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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