Synthesis of novel dimeric compounds containing triazole using click method and their selective antiproliferative and proapoptotic potential via mitochondrial apoptosis signaling
Autor: | Oguzhan Doganlar, Zeynep Banu Doğanlar, Hasan Özyildirim, H. R. Ferhat Karabulut, Ali Osman Karatavuk |
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Rok vydání: | 2020 |
Předmět: |
Lithocholic acid
biology 010405 organic chemistry Organic Chemistry Triazole biology.organism_classification 01 natural sciences Molecular biology 0104 chemical sciences HeLa 010404 medicinal & biomolecular chemistry chemistry.chemical_compound chemistry Apoptosis Cancer cell MTT assay Viability assay General Pharmacology Toxicology and Pharmaceutics Cytotoxicity |
Zdroj: | Medicinal Chemistry Research. 29:643-655 |
ISSN: | 1554-8120 1054-2523 |
Popis: | In this study, the main aim was synthesis of dimeric compounds, which contain lithocholic acid and a triazole structure to investigate the selective cellular and molecular antiproliferative and proapoptotic potential of these products in healthy embryonic fibroblast (MEF), cervix cancer (HeLa), and breast cancer (MCF-7) cells. Four ester (5a–d) and five dimeric (6a–d, 7) out of nine novel compounds were obtained. First of all, lithocholic acid was converted to methyl lithocholate and then it was reacted with certain alkynoic acids (a–d) to obtain its alkynoate derivatives (5a–d). Finally, these compounds were converted to dimers (6a–d) by using 2,6-bis(azidomethyl)pyridine via the click method. Our result indicate that, treatment with dimeric compounds can selectively decrease the cell viability and proliferation in cervix cancer HeLa and breast cancer MCF-7 cells, except 7 which caused a strong cytotoxicity on healthy MEF cells. According to MTT assay, Nucblue cell stain and Annexin V/Propodium iodide molecular probe staining, 100 µM concentrations of the dimeric compounds was sufficient in inducing death and apoptotic cell ratio in HeLa and MCF-7 breast cancer cells selectively. In brief, the present study indicates that most effective dimeric compounds are 6a and 6b, which have the highest IC50 (345.8–342.6 µM) value on healthy cell and the lowest IC50 value in both cervix (49.2–36.9 µM) and breast (23.0–66.1 µM) cancer cells especially long-term treatment and which triggers apoptosis pathway specifically. |
Databáze: | OpenAIRE |
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