Expression and Purification of Isotopically Enriched MHC Binding Immunogenic Peptides for NMR Studies
| Autor: | Edith Weber, Sachin Patil, Lilly M. Saleena, Yong-Wah Kim, Hermann von Grafenstein |
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| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | International Journal of Peptide Research and Therapeutics. 17:137-145 |
| ISSN: | 1573-3904 1573-3149 |
| DOI: | 10.1007/s10989-011-9251-2 |
| Popis: | Peptides are important naturally occurring ligands of MHC molecules. X-ray crystallographic studies have enabled extensive characterization of such peptide ligands. Yet structural and dynamic changes of these peptides in the MHC bound state are not well understood. These conformational transitions are key to understanding the function of MHC molecules and for the development of peptide-based therapeutics. Employing NMR for such studies can fill this gap but it requires the availability of peptides labeled with NMR-active nuclei. Here we report production of nine-mer MHC-binding peptides for use in high resolution NMR studies. The method utilizes a fusion protein approach of attaching the peptide to an easily expressed bacterial protein. The fusion protein construct design allows for rapid purification of the fusion protein and avoids chemical modification of the peptide as a result of the cleavage reaction. The methods developed here allow for rapid cloning of additional MHC binding peptides without significant molecular biology effort. 8–10 mg of mature freeze dried peptides can be obtained from 1 liter of minimal media, sufficient for NMR experimentation. Six uniformly 15N-labeled peptides have been successfully expressed in bacteria and NMR spectra with the expected number of well-resolved signals were recorded. The results obtained here will make peptide-MHC complexes amenable to structural analysis which has not been possible previously. |
| Databáze: | OpenAIRE |
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