Mise Au Point D’un Protocole De Sterilisation D’explants Nodaux D’alchornea Cordifolia Avec De L’acide Triclororoisocyanurique
Autor: | Alexis Nicaise Lepengue, Maurice Ognalaga, Nabil Sabet Mustafa, Christian Moupela, Bertrand M’batchi, Darlène Badjina Eko, Aurélien Mokea-Niaty, Antoine Mitte Mbeang Beyeme, Samson Daudet Medza Mve |
---|---|
Rok vydání: | 2017 |
Předmět: |
021110 strategic
defence & security studies medicine.medical_specialty Alchornea cordifolia biology Chlorothalonil Carbendazim Disinfectant 0211 other engineering and technologies chemistry.chemical_element Hypochlorite 02 engineering and technology 010501 environmental sciences biology.organism_classification 01 natural sciences Surgery chemistry.chemical_compound chemistry Chlorine medicine Trichloroisocyanuric acid 0105 earth and related environmental sciences Nuclear chemistry Explant culture |
Zdroj: | European Scientific Journal, ESJ. 13:274 |
ISSN: | 1857-7431 1857-7881 |
Popis: | Trichloroisocyanuric acid is a swimming pool disinfectant and is readily accessible. As a result, there is the need to use it as a substitute for conventional disinfectants in in vitro culture. Nodal explants of Alchornea cordifolia, harvested in a natural environment, have been rinsed abundantly with Dettol under running water. Then it was soaked in Talo Plus (550 g/l carbendazim and 100 g/l Chlorothalonil) at 5 ml/liter, which is a broad spectrum fungicide. After then, it was immersed in 70% alcohol for 10 minutes before being soaked in different solutions of trichloroisocyanuric acid to: 6, 4, 3, 2, 1, 0.3, 0.1, and 0.08%. The explants were disinfected completely of all contaminating bacterial and fungal exogenous. This was after a treatment in solutions of acidic trichloroisocyanurique of 6 to 0.08%. The results showed that the losses of active chlorine remained low during storage at temperatures of 4 to 18 ± 2°C. They reach only 5.29% after 72 hours. At room temperature of 27 ± 2 ° C, these losses are more than 30% after three days. Concentrations of 0.1 to 0.3% are effective for the disinfection of explants. This protocol of explants disinfection in vitro culture could therefore be advantageously substituted using the hypochlorite of calcium or the chloride of mercury. |
Databáze: | OpenAIRE |
Externí odkaz: |