| Autor: |
Iman S. Gurung, Gwen Tolhurst, Jamie I. Vandenberg, Martyn P. Mahaut-Smith, Juan Martinez-Pinna |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
The Journal of Physiology. 555:61-70 |
| ISSN: |
0022-3751 |
| DOI: |
10.1113/jphysiol.2003.056846 |
| Popis: |
G-protein-coupled receptor signalling has been suggested to be voltage dependent in a number of cell types; however, the limits of sensitivity of this potentially important phenomenon are unknown. Using the non-excitable rat megakaryocyte as a model system, we now show that P2Y receptor-evoked Ca2+ mobilization is controlled by membrane voltage in a graded and bipolar manner without evidence for a discrete threshold potential. Throughout the range of potentials studied, the peak increase in intracellular Ca2+ concentration ([Ca2+]i) in response to depolarization was always larger than the maximal reduction in [Ca2+]i following an equivalent amplitude hyperpolarization. Significant [Ca2+]i increases were observed in response to small amplitude ( |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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