Alkaline phosphatases are involved in the response ofAedes aegyptilarvae to intoxication withBacillus thuringiensissubsp.israelensis Cry toxins
| Autor: | Renaud Stalinski, Laurence Després, Frederic Laporte, Guillaume Tetreau |
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| Rok vydání: | 2016 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine biology Toxin musculoskeletal neural and ocular physiology 030106 microbiology Aedes aegypti musculoskeletal system biology.organism_classification medicine.disease_cause Microbiology Molecular biology Transcriptome 03 medical and health sciences 030104 developmental biology Real-time polymerase chain reaction stomatognathic system Bacillus thuringiensis Gene expression medicine Pathogen Gene Ecology Evolution Behavior and Systematics |
| Zdroj: | Environmental Microbiology. 18:1022-1036 |
| ISSN: | 1462-2912 |
| DOI: | 10.1111/1462-2920.13186 |
| Popis: | Bacillus thuringiensis subsp. israelensis (Bti) is a natural pathogen of dipterans widely used as a biological insecticide for mosquito control. To characterize the response of mosquitoes to intoxication with Bti, the transcriptome profile of Bti-exposed susceptible Aedes aegypti larvae was analysed using Illumina RNA-seq. Gene expression of 11 alkaline phosphatases (ALPs) was further investigated by real time quantitative polymerase chain reaction and ALP activity was measured in the susceptible strain and in four strains resistant to a single Bti Cry toxin or to Bti. These strains were unexposed or exposed to their toxin of selection. Although all resistant strains constitutively exhibited a higher level of transcription of ALP genes than the susceptible strain, they showed a lower total ALP activity. The intoxication with different individual Cry toxins triggered a global pattern of ALP gene under-transcription in all the one-toxin-resistant strains but involving different specific sets of ALPs in each resistant phenotype. Most of the ALPs involved are not known Cry-binding proteins. RNA interference experiment demonstrated that reducing ALP expression conferred increased the survival of larvae exposed to Cry4Aa, confirming the involvement of ALP in Cry4Aa toxicity. |
| Databáze: | OpenAIRE |
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