Sonification as a Means of Enhancing the Detection of Gene Expression Levels from Formalin-Fixed, Paraffin-Embedded Biopsies

Autor:	Thomas Averell Houze, Bengt Gustavsson
Rok vydání:	1996
Předmět:	Reverse transcription polymerase chain reaction Messenger RNA Formalin fixed paraffin embedded law Gene expression Biology Molecular biology General Biochemistry Genetics and Molecular Biology Polymerase chain reaction Reverse transcriptase Biotechnology law.invention
Zdroj:	BioTechniques. 21:1074-1082
ISSN:	1940-9818 0736-6205
Popis:	We describe a novel approach for the isolation and quantification of mRNA from archival specimens by using oligo(dT)25 paramagnetic beads and the reverse transcription polymerase chain reaction (RTPCR). A preliminary study is conducted to evaluate experimental conditions and the effects of varied periods of sonification on the yield of mRNA isolated from formalinfixed, paraffin-embedded human biopsy samples. Validation of this approach will involve the comparison of observed b-actin gene expression levels among different samples from the same patient after various periods of sonification. The novelty of this method is that it offers a means of analyzing archival samples using semiquantitative RT-PCR following sonification-enhanced mRNA extraction. The utility of this protocol allows for gene expression studies to be done effectively using the formalin-fixed, paraffin-embedded archival samples that are commonly available in all hospital pathology departments.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ae0ceec27e30b083c00d78e05c22f9fe https://doi.org/10.2144/96216st05 Zobrazit plný text záznamu