Increased Resistance to Carbapenems inProteus mirabilisMediated by Amplification of theblaVIM-1-Carrying and IS26-Associated Class 1 Integron
| Autor: | Gwendoline Kueffer, Iva Todorova, Angelina Trifonova, Nicolas Kieffer, Patrice Nordmann, Laurent Poirel, Séverine Bontron, Encho Savov |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Pharmacology 0303 health sciences biology 030306 microbiology Immunology biochemical phenomena metabolism and nutrition Integron biology.organism_classification Microbiology Proteus mirabilis Gene dosage DNA sequencing 03 medical and health sciences Gene expression polycyclic compounds biology.protein Gene 030304 developmental biology Carbapenem resistance |
| Zdroj: | Microbial Drug Resistance. 25:663-667 |
| ISSN: | 1931-8448 1076-6294 |
| DOI: | 10.1089/mdr.2018.0365 |
| Popis: | Objective: The aim of the study was to decipher the mechanisms and associated genetic determinants responsible for increased carbapenem resistance among Proteus mirabilis clinical isolates. Methods: The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking, and DNA sequencing. Results: A series of clinical P. mirabilis isolates were consecutively recovered from different patients at the Military hospital of Sofia, Bulgaria. They showed variable levels of resistance to carbapenems. All isolates produced the same carbapenemase VIM-1 that was chromosomally encoded. We showed that increased resistance to carbapenems was related to an increased number of blaVIM-1 gene copies. Conclusion: We showed here that increased carbapenem resistance in P. mirabilis may result from increased expression of the blaVIM-1 carbapenemase gene through multiplication of its copy number. |
| Databáze: | OpenAIRE |
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