| Popis: |
Three new species of the genus Pseudomonas (P. libanensis, P. cedrella, and P. orientalis) isolated from 3 important Lebanese spring waters (Kadicha, Kassam and Mar-Sarkis) in northern Lebanon that feed 45% of the total Lebanese population, were studied for their in vitro virulence factors. We have studied the adhesion, invasion and cytotoxicity properties in HEP-2 cells culture, the production for the following enzymes: proteinase, gelatinase, lipase, lecithinase, DNAse, coagulase, hyaluronidase, chondroitinase, hemolysin, elastase and fibrinolysin, and the acid lability test. All these properties have been studied at 37°C. Two of the P. libanensis strains were cytotoxic, but none of the P. cedrella and P. orientalis strains presents an invasive, adhesive or cytotoxic characters. These new species did not produce a significant enzymes associated with virulence, were not acid resistant. |
