Assessment of the Viability of NIH3T3 Fibroblast Cells Cultured in Polymer Matrices with rhGH
| Autor: | Rogério Miranda Pagnoncelli, Rafaela Radomski da Silva, Cristhiane Alvim Valente, Alexandre da Silveira Gerzson, Denise Cantarelli Machado |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Bone growth
Scaffold Environmental Engineering Materials science Polymers and Plastics Growth factor medicine.medical_treatment 0206 medical engineering technology industry and agriculture Biomaterial Nanotechnology macromolecular substances 02 engineering and technology 021001 nanoscience & nanotechnology 020601 biomedical engineering Andrology PLGA chemistry.chemical_compound chemistry Toxicity Materials Chemistry medicine MTT assay Viability assay 0210 nano-technology |
| Zdroj: | Journal of Polymers and the Environment. 26:906-912 |
| ISSN: | 1572-8900 1566-2543 |
| DOI: | 10.1007/s10924-017-0984-7 |
| Popis: | Developing a biomaterial that functions as a scaffold (osteoconductor), combined with a growth factor (osteoinductor), is of great interest for clinical application in oral and maxillofacial surgery. Growth hormone (GH) is a good candidate, as it is a major regulator of postnatal bone growth and remodeling. Pure PLGA and 70/30 PLGA/PCL matrices were prepared by the solvent evaporation method, combined or not with GH, and tested for toxicity and cell viability using an MTT assay with NIH3T3 mouse cells (ATCC). Cell toxicity was assessed at 24, 48, 72 h, and 7 days of biomaterial exposure to culture medium. All polymers had high cell viability rates. However, from 48 h onwards, the groups with GH-polymer combinations had better results than the polymer groups without association with GH when compared to the control group. At 7 days of culture, only the pure PLGA matrix showed a significant difference from the control group. These results may suggest a preference of cells for the presence of GH in the biomaterial in culture medium, especially in the PLGA matrix. GH appeared to contribute to the increase in cell viability observed at some assessment time points, especially when combined with PLGA as compared to pure PLGA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink