Ion-Pair High-Performance Liquid Chromatographic Determination of Strychnine Alkaloid in an Animal Tissue

Autor:	Carol A. Furcolow, Bruce A. Kimball
Rok vydání:	1995
Předmět:	Detection limit chemistry.chemical_compound Chromatography chemistry Alkaloid Reagent General Chemistry Reversed-phase chromatography Strychnine General Agricultural and Biological Sciences Quantitative analysis (chemistry) Triethylamine High-performance liquid chromatography
Zdroj:	Journal of Agricultural and Food Chemistry. 43:700-703
ISSN:	1520-5118 0021-8561
DOI:	10.1021/jf00051a026
Popis:	A method for the determination of strychnine alkaloid residues in animal tissues using ion-pair reversed-phase high-performance liquid chromatography is described. Following treatment with triethylamine, strychnine was extracted from whole-ground northern pocket gopher tissue with n-butyl chloride. The extraction solvent was evaporated and the sample reconstituted in the liquid chromatographic mobile phase containing the ion-pairing reagent heptanesulfonic acid. This method enables better than 80% strychnine recovery from tissue samples fortified with strychnine at concentrations ranging from 0.2 to 50 μg/g. Excellent precision is also obtained as demonstrated by standard deviations of replicate analyses of approximately 5%. The method limit of detection was 100 ng of strychnine/g of sample
Databáze:	OpenAIRE
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