How Should Apples Be Prepared for a Fruit Salad? A Guided Inquiry Physical Chemistry Experiment

Autor:	Sally S. Hunnicutt, Marc N. Muniz, Robert J. Sweeney, Erica Harvey, Renee Cole
Rok vydání:	2020
Předmět:	Absorbance Science instruction Catechol chemistry.chemical_compound chemistry Physical chemistry General Chemistry Reaction inhibitor Education
Zdroj:	Journal of Chemical Education. 97:4475-4481
ISSN:	1938-1328 0021-9584
DOI:	10.1021/acs.jchemed.0c00517
Popis:	In this guided inquiry experiment, students extract catecholase enzyme from apples to catalyze the oxidation of catechol. They follow the reaction using the UV–vis absorbance of the p-benzophenone produced to determine the Michaelis–Menten kinetic parameters. Students make selected experimental choices within a structured framework such as selecting the apple varietal, the pH of the reaction mixture, and the reaction inhibitor. The experiment has been tested at multiple universities in physical chemistry laboratory courses with both large and small enrollments. We describe the experiment and its implementation in both synchronously and asynchronously taught courses.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::abed790528f7aee49af0f9c74dfeed82 https://doi.org/10.1021/acs.jchemed.0c00517 Zobrazit plný text záznamu