Isolation and identification of potential probiotic bacteria on surfaces of Oreochromis niloticus and Clarias gariepinus from around Kampala, Uganda
Autor: | Samuel Majalija, Andrew Tamale, Nathan Lubowa Musisi, Asuman Sengooba, Ruth Kabarozi, Charles Drago Kato |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Clarias gariepinus Veterinary medicine food.ingredient biology business.industry Aquaculture of tilapia 030106 microbiology Tilapia Plant Science biology.organism_classification Microbiology law.invention Fishery 03 medical and health sciences Oreochromis Probiotic 030104 developmental biology Infectious Diseases food Aquaculture law Lactobacillus business Catfish |
Zdroj: | African Journal of Microbiology Research. 10:1524-1530 |
ISSN: | 1996-0808 |
DOI: | 10.5897/ajmr2016.8235 |
Popis: | Increased fish mortality due to infections has forced most farmers to resort the use of chemotherapeutic agents especially antibiotics. The continued use of these drugs in aquaculture is becoming limited as pathogens develop resistance and infer unpredicted long term public health effects. More research efforts are building to identify alternative disease prevention methods, among which the use of probiotics has been proposed. Therefore, the purpose of this study was to identify potential probiotics on surfaces of tilapia and catfish in areas around Kampala. Tilapia and catfish samples were aseptically collected from selected cages, ponds, tanks and hatcheries around Kampala, including Lake Victoria. The skin of fish was swabbed and then cultured on both general purpose and selective media. Probiotic screening was done using the agar spot method. Results revealed complete growth across all samples. The total microbial load was highest in fish from lakes (1000±9.6×105 cfu) and cages (1001±5.0×105 cfu). In all cases tilapia fish was significantly (p |
Databáze: | OpenAIRE |
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